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Serum Pigment Studies in Newborn InfantsI. Erythroblastosis Fetalis
A. H. TUTTLE, M.D.
AMA Am J Dis Child. 1955;89(5):544-552.
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Serum pigments in infants with erythroblastosis fetalis have been studied spectrophotometrically by Abelson and Boggs1 and by Waters and co-workers.2 Recently Boggs and Abelson3 have determined alkaline hematin levels in erythroblastosis fetalis in an effort to evaluate the severity of the disease. A major difficulty associated with spectrophotometric studies of serum is that withdrawal of blood and separation of plasma or serum is practically always attended by hemolysis to some degree. Hemoglobin thus released may contribute significantly to light absorption, particularly in the region of 415 mµ. We have overcome this difficulty by separating the hemoglobin from other serum pigments by paper electrophoresis, following which spectrophotometric analyses have been done without the interference of hemoglobin.
ANALYTICAL METHODS
Multiple blood specimens were collected for analysis (a) from the umbilical vein at the time of delivery immediately after pulsation had ceased, (b) from the umbilical or femoral vein before,
. . . [Full Text PDF of this Article]
Author Affiliations
Memphis
From the Division of Pediatrics, University of Tennessee College of Medicine, the John Gaston Hospital and Le Bonheur Children's Hospital.
Footnotes
John and Mary R. Markle Foundation Scholar in Medical Science.
Presented in part at the meeting of the Society for Pediatric Research, Buck Hill Falls, Pa., May, 1954.
This investigation was supported in part by a research grant (H-1380) from the National Institutes of Health, Public Health Service.
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