This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Role of Various Diluents and the Dilution Factor in the Interpretation of the X-Ray-Film Test for Fecal Trypsin

DOUGLAS E. JOHNSTONE, M.D.

AMA Am J Dis Child. 1952;84(2):191-198.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

FALSELY positive tests for fecal trypsin due to bacterial gelatinase 1 were found for more than one-half of 137 children² with cystic fibrosis of the pancreas whose feces were tested by the x-ray-film technique.³ Previous studies⁴ have indicated that soybean trypsin inhibitor will inhibit gelatin liquefaction due to pancreatic trypsin and pancreatin-U.S.P. but not that due to bacterial gelatinase. The reliability and consistency of results obtained by the "spot film" technique for fecal trypsin are affected by the following factors: (1) The pH of the fecal suspension¹ (2) Age of the specimen (3) Consistency of the stool (4) Differences in the type of x-ray and photographic film used in the test⁵ (5) Presence of barium and certain other heavy metal ions in high concentration in the fecal specimen⁶ (6) Temperature at which the fecal suspension is incubated on the x-ray film (7) Presence of bacterial gelatinase⁷ . . . [Full Text PDF of this Article]

Author Affiliations
ROCHESTER, N. Y.

From the Departments of Pediatrics, Baltimore City Hospitals and the Johns Hopkins University School of Medicine.

Footnotes
Presented before the Society for Pediatric Research, Atlantic City, N. J., May 3, 1951.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?