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MEASUREMENT BY THE ACETONE METHYLENE BLUE METHOD

C. H. BEST, M.D.; J. H. RIDOUT, M.A.

Am J Dis Child. 1927;34(5):719-720.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

A great variety of methods has been suggested for the assay of solar radiations of wave lengths less than 320 millimicrons. Webster, Hill and Eidinow¹ have used the rate of bleaching of a standard acetone methylene blue solution exposed in a quartz tube. The bleaching² of this solution has been standardized against the lethal dose for Infusoria under certain standard conditions and against the erythema-producing dose for the skin of the average white arm. These authors state that each unit of the methylene blue scale equals from 2 to 4 erythema doses.² It is stated elsewhere³ that two lethal doses for Infusoria are sufficient to produce a mild erythema. The exact limits of the spectrum between which the acetone methylene blue is reduced are not accurately known. Webster, Eidinow and Hill² state that the solution absorbs all rays shorter than 360 millimicrons. Russell and Massengale . . . [Full Text PDF of this Article]

Author Affiliations
TORONTO

From the Section of Physiological Hygiene, School of Hygiene, University of Toronto.

Footnotes
Received for publication, Sept. 9, 1927.

Read at the Thirty-Ninth Annual Meeting of the American Pediatric Society, Chesapeake Bay, May, 1927.

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