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Detection of Tuberculin Sensitivity in Children by Leukocyte Culture
Lillian P. Kravis, MD;
Gordon J. Donsky, MD, FRCP(c);
Harold I. Lecks, MD;
Seema Bhagwat, MSc
Am J Dis Child. 1968;115(2):247-252.
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A NEW approach to the in vitro study of hypersensitivity states in humans unfolded when Nowell published his observations on phytohemagglutinin-induced in vitro transformation of peripheral blood leukocytes.1 For, although Nowell at first was inclined to ascribe this transforming effect to phytohemagglutinin (PHA) 's action on the cell surface, others, in duplicating his findings, likened the transformed cells, apparently derived from the small lymphocyte, to the large pyroninophilic cells seen in antigenically-stimulated lymph nodes or in graft versus host reactions and sought an immunological basis for this mitogenic action.2-5 In attempting to demonstrate the role of hypersensitivity in lymphocyte transformation, Pearmain et al selected tuberculin purified protein derivative (PPD) as a substitute "antigen" for PHA in peripheral-blood leukocyte cultures made from tuberculin-sensitive individuals, and they did, indeed, find that PPD regularly stimulated proliferation and mitotic activity in the cell cultures from Mantouxpositive individuals (with clinically inactive tuberculosis) but
. . . [Full Text PDF of this Article]
Author Affiliations
Philadelphia
From the Department of Allergy, Children's Hospital of Philadelphia.
Footnotes
Received for publication Oct 24, 1967.
Submitted by the authors for the Mitchell I. Rubin Festschrift issue of the JOURNAL.
Reprint requests to 1740 Bainbridge St, Philadelphia 19146 (Dr. Kravis).
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