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Radioreceptor Assay and Radioimmunoassay Comparisons

Stephen LaFranchi, MD; Cheryl E. Hanna, MD; Barbara Jelen

Am J Dis Child. 1984;138(1):23-27.

Abstract
• To investigate possible human growth hormone (HGH) bioinactivity, serum radioreceptor assay (RRA) and radioimmunoassay (RIA) comparisons were made in 48 children undergoing an evaluation for growth retardation. Discrepancies between serum HGH concentrations by RRA and RIA were uncommon; the overall RRA/RIA ratio was 0.75. Significantly reduced HGH RRA/RIA ratios were seen at peak time periods following levodopa and arginine hydrochloride stimulation. Two subjects who demonstrated a discrepancy between serum HGH concentrations by RRA and RIA and somatomedin C levels in the hypopituitary range had malnutrition and chronic disease. There appeared to be an inverse correlation between nutrition, as assessed by a height age—weight age ratio, and RRA/RIA ratios in all subjects. While a reduced RRA/RIA ratio supports the diagnosis of bioinactive HGH, the administration of certain HGH secretagogues, malnutrition, and chronic disease may result in low HGH RRA/RIA ratios.

(AJDC 1984;138:23-27)

Author Affiliations
From the Department of Pediatrics, University of Oregon School of Medicine, Portland.

Footnotes
Read in part at the 64th annual meeting of the Endocrine Society, San Francisco, June 16, 1982.

Reprint requests to Department of Pediatrics, Oregon Health Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201 (Dr LaFranchi).

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