Isolation of cytomegalovirus from cerebrospinal fluid of a congenitally infected infant

R. M. Jamison and A. W. Hathorn Jr

The isolation of cytomegalovirus (CMV) from the CSF of an infant with congenital disease is documented. Samples of urine sediments and CSF were inoculated into tissue cultures of human foreskin fibroblast cells. Cytopathology was evident within 48 hours in those tubes inoculated with CSF. The urine cultures were later positive. All viral isolates were identified by complement fixation tests using known positive CMV antiserum.